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KMID : 1140220140190010007
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대한암예방학회지
2014 Volume.19 No. 1 p.7 ~ p.13
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Anti-Proliferative Effects of Evodiamine in Human Lung Cancer Cells
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Hong Ji-Young
Park So-Hyun
Min Hye-Young
Park Hyen-Joo
Lee Sang-Kook
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Abstract
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Background: Evodiamine, a compound isolated from the Evodia rutaecarpa Bentham (Rutaceae), is known to have a potential anti-proliferative activity in human cancer cells. However, the growth inhibitory activity against lung cancer cells and the underlying molecular mechanisms have been poorly determined. The present study was designed to examine the anti-proliferative effect of evodiamine in A549 human lung cancer cells.
Methods: A549 cells were treated with the compounds from Evodia rutaecarpa , and the anti-proliferative activity was evaluated by the sulforhodamine B assay. The mechanisms of action for the growth inhibitory activity of evodiamine on A549 human lung cancer cells were evaluated by flow cytometry for cell cycle distribution, and by Western blot for the assessment of accumulation and phosphorylation of potential target proteins.
Results: Evodiamine exhibited a potent anti-proliferative activity against A549 human lung cancer cells. Flow cytometric analysis revealed that evodiamine induced cell cycle arrest at G2/M phase and apoptosis in the A549 cells. The cell cycle arrest was well correlated with the decrease of cyclin B1, cyclin A, cdk2 and p-cdc2 (Tyr15) and increase of p-chk1 (Ser345) and p-chk2 (Thr68). Evodiamine also significantly increased the ratio of Bax/Bcl-2 and decreased procaspase-3, suggesting that evodiamine induced apoptosis via the intrinsic apoptotic pathway. In addition, evodiamine inhibited the expression of p-ERK and ERK.
Conclusions: These findings suggest that the anti-proliferative effect of evodiamine is associated in part with the induction of G2/M phase cell cycle arrest and apoptosis, thereby down-regulation of ERK in human lung cancer cells.
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KEYWORD
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Evodiamine, A549 cells, Cell cycle arrest, Apoptosis, Lung cancer
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